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__NOTOC__ | __NOTOC__ | ||
<big>'''Welcome to the NESG Wiki!'''</big><br> | <big>'''Welcome to the NESG Wiki!'''</big><br> | ||
For a more linear view of the contents see [[Wiki Tree Layout]] | For a more linear view of the contents see [[Wiki Tree Layout]] | ||
== Protein | == Protein Sample Production == | ||
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*[[Target selection|NESG target selection]] <br> | *[[Target selection|NESG target selection]] <br> | ||
*[[DNA cloning protocols|DNA cloning protocols]]<br> | *[[DNA cloning protocols|DNA cloning protocols]]<br> | ||
*[[Protein purification|Protein expression and purification protocols]] <br> | *[[Protein purification|Protein expression and purification protocols]] <br> | ||
*Sample Optimization | *Sample Optimization | ||
**[[Construct optimization]] | **[[Construct optimization]] | ||
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*[[Oligomerization Status|Assessment of Oligomerization Status]] | *[[Oligomerization Status|Assessment of Oligomerization Status]] | ||
**[[Gel filtration and light scattering|gel-filtration and light scattering]] | **[[Gel filtration and light scattering|gel-filtration and light scattering]] | ||
**[[NMR determined Rotational correlation time]] | **[[NMR determined Rotational correlation time]] | ||
*[[MassSpectrometry|Mass spectrum]] | *[[MassSpectrometry|Mass spectrum]] | ||
*[[NMR screening]] | *[[NMR screening]] | ||
<br> | <br> | ||
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== NMR Data Acquisition == | == NMR Data Acquisition == | ||
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**[[NMR Sample Preparation]] | **[[NMR Sample Preparation]] | ||
**[[Inserting NMR Sample]] | **[[Inserting NMR Sample]] | ||
**[[Tuning and matching]] | **[[Tuning and matching]] | ||
**[[Deuterium Lock]] | **[[Deuterium Lock]] | ||
**[[Shimming]] | **[[Shimming]] | ||
**[[Pulse width calibration]] | **[[Pulse width calibration]] | ||
**[[Temperature calibration]] | **[[Temperature calibration]] | ||
**[[Chemical shift referencing]] | **[[Chemical shift referencing]] | ||
*Advanced operation | *Advanced operation | ||
**[[Deuterium pulse width calibration and decoupling]] | **[[Deuterium pulse width calibration and decoupling]] | ||
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**[[Simultaneous 13C,15N-resolved NOESY]] | **[[Simultaneous 13C,15N-resolved NOESY]] | ||
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | **[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | ||
**[[Long-range 15N-1H correlation experiments for histidine rings]] | **[[Long-range 15N-1H correlation experiments for histidine rings]] | ||
*[[ | *[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]] | ||
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | **[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | ||
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | **[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | ||
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|} | |} | ||
== NMR | == NMR Data Processing == | ||
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NMRPipe | NMRPipe | ||
*[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions]] | *[[Brief description of philosophy, commands, and functions of NMRPipe|Brief description of philosophy, commands, and functions]] | ||
*[[Routine 2D Experiment|2D experiments]] | *[[Routine 2D Experiment|2D experiments]] | ||
*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments| 3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | *[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | ||
*[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]] | *[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]] | ||
*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]] | *[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]] | ||
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Other | | | ||
Other | |||
*[[Processing NMR spectra with PROSA|PROSA]] | *[[Processing NMR spectra with PROSA|PROSA]] | ||
*[[Bruker Data Processing|TOPSPIN]] | *[[Bruker Data Processing|TOPSPIN]] | ||
*[[AGNuS/AutoProc|AUTOPROC]] | *[[AGNuS/AutoProc|AUTOPROC]] | ||
*[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]] | *[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]] | ||
*[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]] | *[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]] | ||
|} | |} | ||
== NMR Resonance Assignment == | == NMR Resonance Assignment == | ||
*[[Resonance Assignment/Principles and concepts|Principles and concepts]] | |||
{| cellspacing="1" | *[[Resonance Assignment/Principles and concepts|Principles and concepts]] | ||
{| class="FCK__ShowTableBorders" cellspacing="1" | |||
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*Semi-automated protocols | *Semi-automated protocols | ||
**[[Resonance Assignment/CARA|CARA]] | **[[Resonance Assignment/CARA|CARA]] | ||
**[[Sparky]] | **[[Sparky]] | ||
**[[Resonance Assignment/XEASY|XEASY]] | **[[Resonance Assignment/XEASY|XEASY]] | ||
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*Automated resonance assignment | *Automated resonance assignment | ||
**[[AutoAssign|AutoAssign]] | **[[AutoAssign|AutoAssign]] | ||
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**[[Abacus|ABACUS]] | **[[Abacus|ABACUS]] | ||
**[[The PINE Server|PINE server]] | **[[The PINE Server|PINE server]] | ||
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*Validation and deposition | *Validation and deposition | ||
**[[AVS|Assignment validation suite (AVS)]] | **[[AVS|Assignment validation suite (AVS)]] | ||
**[[LACS|Linear analysis of chemical shift (LACS)]] | **[[LACS|Linear analysis of chemical shift (LACS)]] | ||
**[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]] | **[[PDB and BMRB Deposition#Preparing_files_for_BMRB_depostion|Depositing chemical shifts]] | ||
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[[Structure Calculation and Validation|Principles and concepts]] | [[Structure Calculation and Validation|Principles and concepts]] | ||
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Revision as of 15:40, 6 January 2010
Welcome to the NESG Wiki!
For a more linear view of the contents see Wiki Tree Layout
Protein Sample Production
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NMR Data Acquisition
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NMR Data Processing
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NMRPipe |
Other |
NMR Resonance Assignment
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Structure Calculation and Validation
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