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__NOTOC__ | __NOTOC__ | ||
<big>'''Welcome to the NESG Wiki!'''</big><br> | <big>'''Welcome to the NESG Wiki!'''</big><br> | ||
The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR. | |||
Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]] | |||
== Protein Sample Production == | == Protein Sample Production == | ||
{| | {| cellspacing="1" class="FCK__ShowTableBorders" | ||
|- valign="top" | |- valign="top" | ||
| | | | ||
*[[Target selection|NESG target selection]] <br> | *[[Target selection|NESG target selection]] <br> | ||
*[[DNA cloning protocols|DNA cloning protocols]]<br> | *[[DNA cloning protocols|DNA cloning protocols]]<br> | ||
*[[Protein purification|Protein expression and purification protocols]] <br> | *[[Protein purification|Protein expression and purification protocols]] <br> | ||
*Sample Optimization | *Sample Optimization | ||
**[[Construct optimization]] | **[[Construct optimization]] | ||
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*[[SDS page gel]] | *Protein Sample Analysis | ||
*[[Protein concentration|Protein concentration measurements]] | **[[SDS page gel]] | ||
*[[Oligomerization Status|Assessment of Oligomerization | **[[Protein concentration|Protein concentration measurements]] | ||
**[[Gel filtration and light scattering|gel-filtration and light scattering]] | **[[Oligomerization Status|Assessment of Oligomerization State]] | ||
**[[NMR determined Rotational correlation time]] | ***[[Gel filtration and light scattering|gel-filtration and light scattering]] | ||
*[[MassSpectrometry|Mass spectrum]] | ***[[NMR determined Rotational correlation time]] | ||
*[[NMR screening]] | **[[MassSpectrometry|Mass spectrum]] | ||
**[[NMR screening]] | |||
|} | |} | ||
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**[[Pulse width calibration]] | **[[Pulse width calibration]] | ||
**[[Temperature calibration]] | **[[Temperature calibration]] | ||
**[[Chemical shift referencing]] | **[[Chemical shift referencing]] | ||
*Advanced operation | *Advanced operation | ||
**[[Deuterium pulse width calibration and decoupling]] | **[[Deuterium pulse width calibration and decoupling]] | ||
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**[[Simultaneous 13C,15N-resolved NOESY]] | **[[Simultaneous 13C,15N-resolved NOESY]] | ||
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | **[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | ||
**[[Long-range 15N-1H correlation experiments for histidine rings]] | **[[Long-range 15N-1H correlation experiments for histidine rings]] | ||
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]] | *[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]] | ||
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | **[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | ||
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | **[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | ||
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*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | *[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | ||
*[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]] | *[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]] | ||
*[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]] | *[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]] | ||
*[[Alignment Media Preparation|Alignment Media Preparation for RDC measurement]] | |||
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**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]] | **[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]] | ||
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]] | **[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]] | ||
**[[Consensus Approaches|"Consensus" approaches]] | **[[Consensus Approaches|"Consensus" approaches]] | ||
**[[Refinement Strategies|Refinement Strategies]] | |||
| | | | ||
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**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]] | **[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]] | ||
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]] | **[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]] | ||
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]] | **[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]<br> | ||
| | | | ||
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**[[RPF Analysis|RPF analysis]] | **[[RPF Analysis|RPF analysis]] | ||
**[[MolProbity Server|MolProbity server]] | **[[MolProbity Server|MolProbity server]] | ||
**[[RDCvis & KiNG|RDCvis]] | |||
**[[PDB and BMRB Deposition|PDB and BMRB deposition]] | **[[PDB and BMRB Deposition|PDB and BMRB deposition]] | ||
**[[ADIT-NMR|ADIT-NMR]] | **[[ADIT-NMR|ADIT-NMR]] | ||
Latest revision as of 02:29, 29 November 2022
Welcome to the NESG Wiki!
The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.
Please check out NESG NMR wiki workshop presentations at the 2010 Keystone meeting
Protein Sample Production
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NMR Data Acquisition
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NMR Data Processing
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NMRPipe |
Other |
NMR Resonance Assignment
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Structure Calculation and Validation
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