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<big>'''Welcome to the NESG Wiki!'''</big><br>
<big>'''Welcome to the NESG Wiki!'''</big><br>  


The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.
The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.  


Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]]
Please check out [[NESG NMR wiki workshop at the 2010 Keystone meeting|NESG NMR wiki workshop presentations at the 2010 Keystone meeting]]  


== Protein Sample Production  ==
== Protein Sample Production  ==
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**[[NMR screening]]
**[[NMR screening]]


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**[[Pulse width calibration]]  
**[[Pulse width calibration]]  
**[[Temperature calibration]]  
**[[Temperature calibration]]  
**[[Chemical shift referencing]]
**[[Chemical shift referencing]]  
*Advanced operation  
*Advanced operation  
**[[Deuterium pulse width calibration and decoupling]]
**[[Deuterium pulse width calibration and decoupling]]
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**[[Simultaneous 13C,15N-resolved NOESY]]  
**[[Simultaneous 13C,15N-resolved NOESY]]  
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]  
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]]  
**[[Long-range 15N-1H correlation experiments for histidine rings]]
**[[Long-range 15N-1H correlation experiments for histidine rings]]  
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]]
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]]  
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]]  
**[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]]  
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]]
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]]
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**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]  
**[[Structure Calculation Using CS-DP ROSETTA|CS-DP ROSETTA]]  
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]  
**[[Structure Calculation Using CS-RDC-ROSETTA|CS-RDC-ROSETTA]]  
**[[Consensus Approaches|"Consensus" approaches]]
**[[Consensus Approaches|"Consensus" approaches]]
**[[Refinement Strategies|Refinement Strategies]]


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**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]  
**[[Structure Refinement Using CNS Energy Minimization With Explicit Water|CNS refinement]]  
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]  
**[[Structure Refinement Using XPLOR-NIH|XPLOR-NIH refinement]]  
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]
**[[Rosetta High Resolution Protein Structure Refinement Protocol|ROSETTA refinement]]<br>


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**[[RPF Analysis|RPF analysis]]  
**[[RPF Analysis|RPF analysis]]  
**[[MolProbity Server|MolProbity server]]  
**[[MolProbity Server|MolProbity server]]  
**[[RDCvis & KiNG|RDCvis]]
**[[PDB and BMRB Deposition|PDB and BMRB deposition]]  
**[[PDB and BMRB Deposition|PDB and BMRB deposition]]  
**[[ADIT-NMR|ADIT-NMR]]  
**[[ADIT-NMR|ADIT-NMR]]  
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For a more linear view of the contents including those in development see [[Wiki Tree Layout]]

Latest revision as of 02:29, 29 November 2022


Welcome to the NESG Wiki!

The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.

Please check out NESG NMR wiki workshop presentations at the 2010 Keystone meeting

Protein Sample Production


NMR Data Acquisition

NMR Data Processing

NMRPipe

Other

NMR Resonance Assignment

Structure Calculation and Validation

Principles and concepts