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*[ | *[[Target selection|NESG target selection]] <br> | ||
*[[DNA cloning protocols|DNA cloning protocols]]<br> | *[[DNA cloning protocols|DNA cloning protocols]]<br> | ||
*[ | *[[Protein purification|Protein expression and purification protocols]] <br> | ||
*Sample Optimization | *Sample Optimization | ||
**[[Construct optimization]] | **[[Construct optimization]] | ||
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**[[NMR screening]] | **[[NMR screening]] | ||
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**[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]] | **[[NMR experiment setup scripts for VNMRJ|Custom NMR experiment setup scripts for VNMRJ]] | ||
**[[Estimation of rotational correlation time]] | **[[Estimation of rotational correlation time]] | ||
**[ | **[[Estimation of measurement time]] | ||
**[[Simultaneous 13C,15N-resolved NOESY]] | **[[Simultaneous 13C,15N-resolved NOESY]] | ||
**[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | **[[2D (13C, 1H) HSQC for fractionally 13C-labeled samples|2D [13C, 1H]-HSQC for fractionally 13C-labeled samples]] | ||
**[ | **[[Long-range 15N-1H correlation experiments for histidine rings]] | ||
*[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]] | *[[Setting up non-uniformly sampled spectra|Non-uniform sampling (NUS) ]] | ||
**[ | **[[Setting up non-uniformly sampled spectra/NUS guide for Varian|NUS - Varian]] | ||
**[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | **[[Setting up non-uniformly sampled spectra/NUS guide for Bruker according to Arrowsmith group in Toronto|NUS - Bruker]] | ||
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*Maintenance (VARIAN) | *Maintenance (VARIAN) | ||
**[[Installing and updating BioPack]] | **[[Installing and updating BioPack]] | ||
**[ | **[[Full probefile calibration]] | ||
**[ | **[[Rebooting spectrometer console]] | ||
**[ | **[[Conditioning procedure for cryogenic probes]] | ||
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*[[Routine 2D Experiment|2D experiments]] | *[[Routine 2D Experiment|2D experiments]] | ||
*[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | *[[Routine Processing Procedure for 3D 15N and 13C-edited Experiments|3D <sup>15</sup>N and <sup>13</sup>C-edited experiments]] | ||
*[ | *[[HSQCTROSY RDC Measurement|2D ]]<sup>[[HSQCTROSY RDC Measurement|15]]</sup>[[HSQCTROSY RDC Measurement|N HSQC-TROSY experiment for RDC measurement]] | ||
*[ | *[[Jmodulation Experiment RDC|2D J-modulation experiment for RDC measurement]] | ||
*[ | *[[Alignment Media Preparation|Alignment Media Preparation for RDC measurement]] | ||
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*[[Processing NMR spectra with PROSA|PROSA]] | *[[Processing NMR spectra with PROSA|PROSA]] | ||
*[[Bruker Data Processing|TOPSPIN]] | *[[Bruker Data Processing|TOPSPIN]] | ||
*[ | *[[AGNuS/AutoProc|AUTOPROC]] | ||
*[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]] | *[[Processing non-uniformly sampled spectra with Multidimensional Decomposition|Processing NUS spectra with MDD]] | ||
*[ | *[[Spectra Format Conversion from NMRPipe Data|NMRPipe processed data conversion to Sparky, CARA, XEASY, and NMRViewJ]] | ||
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Latest revision as of 02:29, 29 November 2022
Welcome to the NESG Wiki!
The NESG Wiki is a medium for sharing experimental protocols as well as training an educational materials in the fields of structural biology, structural genomics and biomolecular NMR.
Please check out NESG NMR wiki workshop presentations at the 2010 Keystone meeting
Protein Sample Production
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NMR Data Acquisition
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NMR Data Processing
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NMRPipe |
Other |
NMR Resonance Assignment
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Structure Calculation and Validation
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